Part:BBa_K133031:Design

HpaA-CF-RGD-Histop

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 139
  Illegal NheI site found at 1993
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 170
  Illegal BglII site found at 266
  Illegal BamHI site found at 1550
  Illegal BamHI site found at 2061
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1087
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2344
  

Design Notes

The part was cloned into pSB1.AK3 without terminator for use in bacterial cells (surface expression).

Source

HpaA was ordered from GeneArt, CF was from a plasmid, RGD-Hisstop was made with annealing